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. 2021 Jun 7;11(6):852. doi: 10.3390/biom11060852

Figure 3.

Figure 3

Jumonji 30 demethylase is sufficient for the removal of histone H3K27me3 from the HSP21 gene before heat shock and affects H3K4me3 levels. (a) Schematic representation of the temperature conditions used. Timing of ChIP–qPCR is denoted by the red dot. Orange and magenta lines show two different timings of β-estradiol application. Transgenic plants (pER8:JMJ30) in the jmjq mutant background subjected to β-estradiol application before acclimation (ACC) (①, left) and before heat shock (HS) (②, right). (b–d) H3K4me3 (b), H3K27me3 (c), and histone H3 (d) levels at the HSP21 gene in the wild type and jmjq mutants as determined by ChIP–qPCR. Gray jitter dots represent expression level in each sample. Asterisks indicate significant differences based on one-way ANOVA test (* p < 0.05). The letters above the bars indicate significant differences, while the same letters indicate non-significant differences (post-hoc Tukey’s HSD test (p < 0.05)). NS, not significant.