Figure 2.

Interactions between PODXL and its intracellular binding partners’ ezrin and NHERF1/2. The intracellular domain of PODXL contains several phosphorylation sites and a C-terminal DTHL motif, which can directly interact with with the N-terminal FERM domain of ezrin. In addition, ezrin can interact with PODXL indirectly via the C-terminal of NHERF1/2, following the binding of the DTHL motif of PODXL to PDZ2 domain of NHERF1/2. Activation of the PODXL-NHERF-1/2-ezrin complex results in subsequent binding to the actin cytoskeleton. Sequestration of RhoGDI by ezrin releases Rho-GDP, which converts to Rho-GTP to enhance the activation of Rho-family GTPases. Specific protein-protein interactions are indicated by the black arrows. Adapted from McNagny and Hughes [20].