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Effect of melatonin on the viability of human GBC cells. The Cell Counting Kit-8 assay was performed to assess the effect of melatonin on the viability of GBC cells treated with melatonin (0–3 mM) for (A) 24 or (B) 48 h. (C) GBC cells were treated with melatonin (0–3 mM) for 48 h and subsequently subjected to flow cytometry to detect apoptosis. GBC, gallbladder cancer; OD, optical density.
