Figure 3.

Competition experiments with the other natural products synthesized, and goyazensolide inhibits the interaction of IPO5 with specific NLSs. (a) SW620 lysate was incubated for 30 min with DMSO or one of the natural products (10 μM, 30 min) and then labeled with 10 μM GOYA-2 followed by a CuAAC reaction with Cy3–N₃ for 1 h at RT in the dark and analyzed by SDS-PAGE using fluorescence as a readout. The labeling/competition experiment shows selective binding of goyazensolide (1) and atripliciolide (2) to IPO5. (b) Left, sequence of two viral biotinyleted NLSs, NLS-1 (HIV Rev) and NLS-2 (PB1 influenza A). Right, immunoblot of competitive interaction between NLS and IPO5. Magnetic streptavidin beads were saturated with corresponding NLSs and then incubated for 2 h with HT29 lysate treated with DMSO or goyazensolide (10 μM, 30 min).