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. 2021 May 25;7(6):954–962. doi: 10.1021/acscentsci.1c00056

Figure 4.

Figure 4

Covalent engagement of IPO5 with goyazensolide inhibits its transport activity in cells. (a) Illustration of IPO5 involvement in the Ras pathway. (b) Confocal microscopy images of SW620 cells treated with DMSO or 1 or 2.5 μM goyazensolide for 3 h, imaging of localization of RASAL2 using an RASAL2 primary antibody followed by an Alexa fluor488 secondary antibody (green), nuclear stain with Hoechst (blue), and fluorescence quantification of the images. Fluorescence intensity profile across the red line is shown on the right. (c) Ratio of fluorescence in cytosol versus nucleus (number of cells > 15). (d) Immunoblot of p-AKT (p-AKT Ser473 antibody) expression upon treatment of SW620 cells with DMSO or goyazensolide (5 μM, 4 h).