Figure 4.
Loop-mediated isothermal amplification (LAMP) lower limit of detection study. (a) 12% acrylamide gel of digested HPV 18 LAMP amplicons; stochastic amplification occurs below 104 cp/μL of input HPV 18 plasmid DNA. (b) Only samples that contained positive HPV 18 LAMP amplicons, as determined by gel electrophoresis, resulted in a signal decrease in methylene blue on the gold leaf electrodes below a given threshold. (c) Probit analysis of the electrochemical data indicates that the lower limit of detection for the electrochemical detection of LAMP amplicons is 1.2 × 104 total copies of HPV 18 DNA.