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. 2021 Jun 2;11(6):831. doi: 10.3390/biom11060831

Figure 2.

Figure 2

Single protein maximum likelihood tree of CMAH protein sequences from various species. Sponge CMAH proteins form a clade and are grouped together with other metazoan sequences. Sequences were aligned using MUSCLE [29], trimmed in Geneious 8.1.9 (BioMatters, Ltd., Auckland, NZ) [30] and the tree was made in IQTree v2.1.2 [31] with the substitution model (LG+I+G4) selected using ModelFinder [32]. Nonparametric bootstrap branch supports are shown next to the nodes. As evident from the non-basal position of cnidarians, single protein trees only approximate true phylogenetic relationships.