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. 2021 Jun 7;10(6):924. doi: 10.3390/antiox10060924

Figure 3.

Figure 3

Figure 3

Expression of ARE-dependent transcription by Nrf2/Cnc in several types of neurons in the central adult brain. (A,E,I,M,P,S) Confocal microscopy observation of GFP fluorescence monitoring ARE-dependent transcription by Nrf2/Cnc transcription factor in five types of neurons and mushroom bodies in brains from adults fed on sesamin. The glutamatergic neurons (A–D), cholinergic neurons (E–H), dopaminergic neurons (I–L), serotonergic and dopaminergic neurons (M–O), GABAnergic neurons (P–R), and mushroom bodies (S–U) were labelled by RFP using VGlut-Gal4 (VGlut > RFP), ChAT-Gal4 (ChAT > RFP), TH-Gal4 (TH > RFP), Ddc-Gal4 (Ddc > RFP), Gad1-Gal4 (Gad1 > RFP), and GawB-Gal4 (4G > RFP) respectively. Images in (A,E,I,M,P,S) correspond to clusters of each type of neurons in brains from flies fed on the diet with 0.5% DMSO (control), or with 2 mg/mL sesamin in 0.5% DMSO (sesamin). Scale bars in (A,E,M,P) represent 10 μm, a bar in I represents 20 μm, and a bar in S represents 50 μm. (B,F,J) The total area showing ARE-GFP fluorescence (n ≥ 10). (C,G,K,N,Q,T) Ratios of the areas among ARE and RFP-positive areas in brains (n ≥ 4) were calculated by ImageJ for each condition. (D,H,L,O,R,U) Intensity of the GFP fluorescence was calculated by ImageJ for each condition (n ≥ 9). ns not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, Student’s t-test.