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. 2021 Jun 7;10(6):924. doi: 10.3390/antiox10060924

Figure 5.

Figure 5

Suppression effects of sesamin on the oxidative stress-induced reduction of areas occupied by glutamatergic neurons in adult brains harboring the neuron type-specific depletion of Sod2. (A) In glutamatergic neurons labelled in red by RFP expression, the neuron-specific depletion of Sod2 mRNA was carried out (VGlut > RFP, Sod2RNAi). Only a basal level expression of VGlut > RFP was observed in clusters of glutamatergic neurons in male brains (A1) and female brains (A3) from flies fed on the control diet, while feeding of the diet with sesamin at 2 mg/mL enhanced the RFP fluorescence in both male (A2) and female brains (A4). (B,C) Total area occupied by the cholinergic neurons in the brain (VGlut > RFP) (B), or in brains harboring glutamatergic neuron-specific ROS accumulation due to the Sod2 depletion (VGlut > RFP, Sod2RNAi) (C), from adults (n ≥ 12 each). Brains were prepared from the male or female flies fed on a diet either with 0.5% DMSO only (control), or with sesamin at 2 mg/mL in 0.5% DMSO (sesamin) for 7 days. Scale bar represents 10 μm. Student’s t-test, ns not significant, * p < 0.05.