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. 2021 Jun 8;9(6):620. doi: 10.3390/vaccines9060620

Figure 2.

Figure 2

FMDV particle- and strain-specificity of VHHs. DAS-ELISAs were performed using a matrix of 32 VHHs and 21 FMDV strains representing four serotypes. Each strain was used in ELISA at 1 µg/mL 146S either as untreated authentic particles (blue) or after conversion into 12S by heating (red). The absorbance value at 450 nm after subtraction of the background absorbance without FMDV particles is shown. The 12 VHHs of the 6 CDR3 groups comprising the 10 VHHs that bind specifically to 146S of serotype A strains are color-coded by their CDR3 group. The control VHHs isolated earlier are underlined. The VHHs are arranged from top to bottom according to their CDR3 group and their particle and strain specificity. Broadly reactive control VHH M8F, however, is placed on top to show all FMDV particle preparations contain immunoreactive material. FMDV strains are arranged from left to right according to their phylogenetic relationship (Figure 3).