Figure 6.

VHH binding to wild-type and stabilized mutant VLPs of various serotypes. The authentic particles (APs) or the different types of VLPs were either untreated or heated at 56 °C for 1 h as indicated on top of each column of panels. The strain used is indicated on the left of each row of panels. Different particles were two-fold titrated in DAS-ELISAs with a 2 µg/mL starting concentration. The control VHHs M8F and M311F, which bound all types of particles, are shown by a yellow curve in each panel. The 12S specific control VHH M3F is shown by a dark blue curve.