Table 2.
Sequence and phage display selection information of 26 newly isolated VHHs.
| VHH a | VHH Subfamily b | Phage Display Selection | CDR3 Sequence | VHHs from Same CDR3 Group (Number of Amino Acid Differences) d | |
|---|---|---|---|---|---|
| FMDV Strain c | Capture VHH | ||||
| M643 | 1 | A/TUR/14/98 | M8F | ATDPSWTAFKNAARYDY | M659(17), M702(14), M652(13) |
| M652 | 1 | A/TUR/14/98 | M8F | ATDQSWTASKNAARYDN | M659(12), M702(13), M643(13) |
| M659 | X | A22/IRQ/24/64 | M321F | AADQSWTASMNPARYDY | M702(15), M643(17), M652(12) |
| M702 | 1 | A22/IRQ/24/64 | M321F | AADPSWTASMNAARYDY | M659(15), M643(14), M652(13) |
| M691 | X | A24/Cruzeiro/BRA/55 | M326F | KLEDWITGQTY | M703(9) |
| M703 | 2 | A/TUR/14/98 | M8F | NLKDWITGQTY | M691(9) |
| M686 | 1 | A/TUR/14/98 | M8F | AAKYKSTFYTTMDVQYDY | M688(7) |
| M688 | 1 | A/TUR/14/98 | M8F | AAKYQSTFYSTMDVQYDY | M686(7) |
| M669 | X | A/TUR/14/98 | M8F | TARGWDDTEYPY | M676(8) |
| M676 | X | A/TUR/14/98 | M8F | VGRGWDDTEYPY | M669(8) |
| M677 | 1 | A/TUR/14/98 | M8F | AAGWSGMAPSRANSYAY | NA |
| M678 | 2 | A/TUR/14/98 | M8F | NTVRRVATLSGSSSGS | NA |
| M651 e | 1 | A22/IRQ/24/64 | M321F | AVEEDSGLNYDAGWYDY | NA |
| M661 | 1 | A/TUR/14/98 | M8F | AADNAVVVPTTTRGYDY | NA |
| M655 e | 1 | A24/Cruzeiro/BRA/55 | M326F | AARTSPYYSAYASHYDY | NA |
| M662 | 1 | A24/Cruzeiro/BRA/55 | M326F | AASGSYNDFFEDDVDY | NA |
| M679 | 1 | A/TUR/14/98 | M8F | AAREGPAIYNSDRYFPY | NA |
| M675 | 1 | A22/IRQ/24/64 | M321F | AAREDYYYTGHEYDY | NA |
| M685 | 1 | A22/IRQ/24/64 | M8F | AADLDRWESVATMIYDYDY | NA |
| M658 | 1 | Asia1/Shamir/ISR/89 | M98F | AAAWSFRSDYGARIKSAYDF | M332(5) |
| M684 | 1 | A22/IRQ/24/64 | M321F | AVGRSLVRDSRAYDY | NA |
| M642 | 2 | Asia1/Shamir/ISR/89 | M98F | NARATSGWASYVT | NA |
| M665 | 2 | O/TAW/3/97 | M8F | YAGEAAGWGSHVY | NA |
| M664 | 2 | O/TAW/3/97 | M23F | NVRDQAGLGYSDY | NA |
| M663 | 1 | A24/Cruzeiro/BRA/55 | M326F | TAGRSYSSNPAAYNY | NA |
| M680 | 1 | A24/Cruzeiro/BRA/55 | M326F | AADRSLTTRDVAYDY | NA |
a The 12 VHHs of the 6 CDR3 groups comprising the 10 VHHs that bind specifically to 146S of serotype A strains are color-coded by their CDR3 group in purple, green, orange, red, gray and magenta (VHHs in black belong each to a unique CDR3 group). b Subfamilies were earlier defined [31]. X indicates VHHs that could not be classified. c The underlined strain indicates that 12S from another strain was used as a competitor in panning. d All clones from different CDR3 groups showed at least 20 amino acid differences, except M665 and M642 that showed 13 amino acid differences. NA, not applicable. e M651F and M655F are the only two VHHs containing a potential N-glycosylation site. The Asn residue of the potential N-glycosylation site was located at IMGT positions 57 and 84, respectively.