Table 1.
Oligonucleotide sequence of splenic gene primers.
| Gene 1 | Sequence (5′-3′) | Annealing Temperature (°C) | Product Size (bp) | Accession Number | Reference |
|---|---|---|---|---|---|
| IL4 | F-AATGACATCCAGGGAGAGGTTTC R-GCTAGTTGGTGGAAGAAGGTACG |
55 | 219 | JN639847 | [3] |
| TNFα | F-AGACCAGATGGGAAGGGAATGAA R-GAAGAGGCCACCACACGACAG |
55 | 219 | JN942589 | [8] |
| INFγ | F-AGCTGACGGTGGACCTATTATTGT R-CGGCTTTGCGCTGGATTC |
58 | 260 | JN942588 | [3] |
| TLR2 | F-GTGGCCATGTCGATCAGCAGAAAC R-TCAGCGGAGAGTCACAGATGTAGC |
56 | 202 | NM_204278 1 | [8] |
| 28S rRNA | F-CAGGTGCAGATCTTGGTGGTAGTA R- GCTCCCGCTGGCTTCTCC |
58 | 273 | JN639848 | [3] |
1 IL4, interleukin 4; TNFα, tumor necrosis factor alpha; INFγ, interferon gamma; TLR2, Toll-like receptor 2.