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The number of mice defecating during different methods of identification marking. The treatments (n = 12) were as follows: EP = ear-punch; EP+A = ear-punch plus anaesthetic cream; MP = permanent marker on the tail. EP and EP+A was only conducted once per mouse, but MP was repeated weekly. MP mice were significantly more likely to defecate at a first application than at b subsequent applications (p < 0.001).
