Skip to main content
. 2021 May 27;10(6):862. doi: 10.3390/antiox10060862

Figure 1.

Figure 1

Cardamonin suppressed IL-1β-induced iNOS and COX2 expression and prevented the IL-1β-mediated induction of PGE2 and the IL-1β-mediated production of nitric oxide in human OA chondrocytes. (A) MTT assays were used to examine the cytotoxicity of cardamonin on human chondrocytes incubated with 0, 1, 5, 10, 20, and 40 µM/mL of cardamonin for 24 or 48 h. (B–D) At a concentration of 10 µM, cardamonin was shown to significantly reduce iNOS and COX-2 protein expression levels in chondrocytes stimulated with IL-1β for 24 h. (E,F) PGE2 and nitric oxide concentrations were significantly increased following treatment with IL-1β for 24 h; however, these increases were significantly decreased following treatment with 10 µM cardamonin. All data comes from at least three independent experiments, and their significance were as follows: cardamonin group and the control group are indicated by an asterisk (*); cardamonin group and the IL-1β group are indicated by a hash symbol (#). # p < 0.05; **, ## p < 0.01; *** p < 0.001.