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Detection of LSDV DNA in clinical specimens of affected cattle by the GPCR gene targeting PCR and LSDV specific antibody in ELISA.
| Subject | Vaccination Status 1 | PCR Results with Specimens from | Detection of DNA | ELISA | |||
|---|---|---|---|---|---|---|---|
| Blood | Nose | LSDV | SPPV | GTPV | |||
| Case1 | vaccinated | + | + | + | − | − | + |
| Case2 | vaccinated | + | + | + | − | − | + |
| Case3 | unvaccinated | + | + | + | − | − | − |
| Cow4 (negative control) | vaccinated | − | − | − | − | − | + |
1 SPPV-based vaccine was used for the immunization of the cattle; ‘+’—positive reaction; ‘−’—negative reaction.
