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. 2021 May 27;11(6):966. doi: 10.3390/diagnostics11060966

Figure 3.

Figure 3

Hemagglutination inhibition based on the conventional tube method. A total of 10 serum samples (A–J) were treated with varying concentrations of Hi5 cells expressing Gp.Mur antigen (1.0 × 105, 5.0 × 104, or 2.5 × 104). As a control, we used WT-td-infected Hi5 cells (1.0 × 105). Evaluation of levels of hemagglutination revealed a dose-dependent hemagglutination inhibitory response due to anti-Gp.Mur antibody in the sera. Samples (A–I) were tested at the IS phase, whereas sample (J) was tested at the AHG phase. The 0/mf indicates presence of small agglutinates among nonagglutinated cells.