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. 2021 Jun 2;13(6):1055. doi: 10.3390/v13061055

Figure 2.

Figure 2

Mapping minimum region and critical residues for RBD:ACE2 PPI. (A) Schematic of RBD deletions. RBD, receptor binding domain; SP, signal peptide; NTD, N-terminal domain; RBM, receptor binding motif. (B,E,G) Western blot analysis of protein expression. (C,F,H) In vitro luciferase assay. Equal amounts (1 µg) of each biosensor component were incubated together for 30 min, followed by luciferase assay; (D) Schematic of ACE2 deletions. Experimental procedures and labels are as described in Figure 1. *, p < 0.05.