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. 2021 Jun 2;13(6):1055. doi: 10.3390/v13061055

Figure 4.

Figure 4

Mutations of SARS-CoV-2 RBD on its interaction with ACE2 and response to antibody and peptides. (A) Western blot analysis of wild-type (WT) and mutant RBD-Lg-His. (B) Mutations of RBD on its ACE2-binding ability. In vitro luciferase assay was carried out as described in Figure 1E. (C) Mutations of RBD on their response to antibody. Protein lysates extracted from WT or mutant RBD-Lg-His were preincubated with 50 µg/mL of IgG or VHH72, and subsequently incubated with Sm-ACE2 lysate, followed by luciferase assay. (D) Mutations of RBD on their response to peptide inhibition. Experimental procedures and data analysis are as described in Figure 3D. *, p < 0.05.