Figure 2.

Intracellular levels of free thiols after benznidazole and hemin preincubation. A fluorescent probe was employed to indirectly measure the free thiol levels after 3 h preincubation with 0.5 mM or 1.0 mM benznidazole or 0.2 mM hemin. Bars represent the mean + SEM of thiol-conjugated methylfluorescein (TMF) MFI in the (A) Y strain and (B) Y-R^Bz parasites compared to respective controls (CTL). The values of significance were represented by (**) for p < 0.01 and (***) p < 0.001, n = 3 (Y) and n = 5 (Y-R^Bz) independent experiments.