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CF accumulation after sphingosine preincubation. ABCC-like activity was evaluated by the CF efflux assay after a preincubation with 60 µM sphingosine for 1 h. (A) Representative histograms, (B) CF MFI, and (C) percentages of CF+ parasites from Y strain. Lines represent the median and values of significance were represented by (*) for p < 0.05, n = 6 independent experiments.
