Figure 8.

Viability of parasites after benznidazole treatment and thiol depletion. The viability was measured by staining parasites with 1 µg/mL propidium iodide (PI) for 15 min after incubation with benznidazole alone or in combination with 200 µM MK-571 (A,B) or 3 mM buthionine sulfoximine (BSO) (C,D) for 24 h. (A,C) Percentages of PI- parasites (viable cells) from Y strain and (B,D) Y-R^Bz parasites. Bars represent the mean + SEM and the values of significance were represented by (*) for p < 0.05, (**) p < 0.01, and (***) p < 0.001, n = 9 (Y) and n = 7 (MK-571/Y-R^Bz) and n = 5 (BSO/Y-R^Bz) independent experiments.