Figure 6.

Fluorescence microscopy images of living Candida albicans cells pre-treated with lucifer yellow (LY) and propidium iodide (PI), then treated with R10S-RR. (a) Light transmission; (b) 20 min after the addition of R10S-RR peptide, LY is evidenced inside many yeast cells, suggesting an irreversible peptide-induced membrane permeabilization resulting in cell death, as demonstrated by PI internalization (c). Arrows in panels (b) indicate LY localization at the cell wall level prior to internalization. The inset shows a detail of the field highlighted by the hatching. (d) Merge of (b,c). Bar, 10 μm.