Figure 3.

Phylogenetic tree based on nucleotide sequences of the flagellin gene (466-bp) of Candidatus Borrelia javanense in ectoparasite ticks, A. javanense, from M. javanica pangolins and the comparison sequences. Underline indicates the Candidatus B. javanense identified in this study; GenBank accession numbers are provided for all isolates. The neighbor-joining method was used to construct the evolutionary tree. The percentage of replicate evolutionary trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the maximum composite likelihood method and are in the units of the number of base substitutions per site (MEGA7, 2015).