Table 1.
Primers used for Borrelia-specific 16S rRNA and flaB gene amplification in this study, including primer sequences, annealing temperature, and expected product size.
| Gene | Primer | Sequence (5′-3′) | Annealing Temperature | Expected Product Size | Reference |
|---|---|---|---|---|---|
| 16S rRNA | External | ||||
| Bor-0F | AAAATAACGAAGAGTTTGATCCTGG | This study | |||
| Bor-1533R | GTGATCCAGCCACACTTTCCAGTA | 55 °C | 1533 bp | This study | |
| Internal | |||||
| Brm1 | CGCTGTAAACGATGCACACTTGGTGTTAATC | [26] | |||
| Brm2 | CGGCAGTCTCGTCTGAGTCCCCATCT | 60 °C | 353 bp | [26] | |
| Brm1-R | GATTAACACCAAGTGTGCATCGTTTACAGCG | 55 °C | 790 bp/Bor-0F | This study | |
| flaB | External | ||||
| FLA120F | AGAATTAATMGHGCWTCTGATGATG | [26] | |||
| Flab764R | GCATCTTCGATCTTTGAAAGTGACATATT | 55 °C | 645 bp | This study | |
| Internal | |||||
| Flab625R | CTGGAGCTGCTTGAGCACCTTCT | 55 °C | 506 bp/FLA120F | This study |