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. 2021 May 28;10(6):1343. doi: 10.3390/cells10061343

Figure 4.

Figure 4

Thymosin β4 activates capsulin positive progenitors in uninjured hearts and initiates organ-wide epicardial thickening at the non-infarcted areas of the infarcted adult mice heart in vivo. (af), Thymosin β4 administration (ac) activates perivascular (c white arrowheads) and vascular (c black arrowhead) capsulin positive cells when compared to PBS controls (df). (a,d) are frontal, (b,e) are dorsal views of representative thymosin β4 or PBS treated hearts. (c,f) are high power images of the boxed areas in (b,e). (gj), Immunohistochemical analysis with anti-Bves antibody shows significant increase in Bves-positive cells of epicardial origin and organ-wide thickening of the epicardium along with a significant increase of the number of capillaries and mature vessels 3 days after systemic thymosin β4 treatment (g) compared to PBS (i). (h,j) are DAPI stain of (g,i). ec: epicardium.