Table 1.
List of differentially up-regulated proteins with statistical significance (p < 0.05) in BRAFV600E mutant HT-29 cells in comparison to Caco-2 and SW480 colon cancer cells.
| Spot Number | Accession ID | Protein Name p-Value (HT-29 vs. Caco-2; HT-29 vs. SW480) |
Molecular Weight (kDa) | Peptide Matches | Sequence Coverage (%) | SCORE |
|---|---|---|---|---|---|---|
| 160 | MIC60_HUMAN | MICOS complex subunit MIC60 (0.002; 0.044) |
83.60 | 4 | 10.00 | 307.41 |
| 517 | K1C20_HUMAN | Keratin, type I cytoskeletal 20 (0.001; 0.001) |
48.50 | 4 | 9.90 | 145.21 |
| 678 | ROAA_HUMAN | Heterogeneous nuclear ribonucleoprotein A/B (0.010; 0.011) |
36.20 | 2 | 3.90 | 60.25 |
| 731 | NPM_HUMAN | Nucleophosmin (0.028; 0.016) |
32.60 | 4 | 16.30 | 191.18 |
| 717 | K2C1_HUMAN | Keratin, type II cytoskeletal 1 (0.009; 0.003) |
66.00 | 1 | 2.50 | 44.11 |
| 925 | TPM1_HUMAN | Tropomyosin alpha-1 chain (0.030; 0.001) |
32.70 | 2 | 8.80 | 79.33 |
| 983 | RANG_HUMAN | Ran-specific GTPase-activating protein (0.001; 0.0003) |
23.47 | 16 | 47.00 | 76.00 |
| 996 | UCHL3_HUMAN | Ubiquitin carboxyl-terminal hydrolase isozyme L3 (0.039; 0.023) |
26.34 | 18 | 70.00 | 178.00 |
| 1191 | ARI5B_HUMAN | AT-rich interactive domain-containing protein 5B (0.006; 0.002) |
133.43 | 14 | 17.00 | 37.00 |
| 985 | MIXTURE 1 SERB_HUMAN IF4E_HUMAN |
Phosphoserine phosphatase Eukaryotic translation initiation factor 4E (0.040; 0.030) |
25.16 25.31 |
16 14 |
62.00 48.00 |
100.00 85.00 |
| 961 | YWHAZ _HUMAN | 14-3-3 protein theta (0.043;0.061) |
28.032 | 13 | 40.00 | 53.00 |
| 1162 | NMES1_HUMAN | Normal mucosa of oesophagus-specific gene 1 protein (0.005; 0.004) |
9.611 | 4 | 55.00 | 37.00 |
| 855 | ENOPH_HUMAN | Enolase-phosphatase E1 (0.027; 0.0978) |
28.90 | 6 | 34.10 | 378.18 |
| 971 | YWHAQ_HUMAN | 14-3-3 protein zeta/delta (0.073; 0.043) |
27.899 | 21 | 38.00 | 114 |
| 655 | CAPG_HUMAN | Macrophage-capping protein (0.0003; 0.077) |
38.50 | 4 | 10.30 | 223.83 |