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. 2021 Jun 1;11(6):827. doi: 10.3390/biom11060827

Figure 4.

Figure 4

Stopped-flow and time-dependent fluorescence spectroscopy measurements of the dissociation of the WT TCF7L2–β-catenin complex. First, a 200 nM fluorescent-labelled WT TCF7L2–β-catenin complex was mixed in with 50-times molar excess of unlabelled WT TCF7L2 (1–54), and the reaction was monitored by (A) stopped-flow fluorescence and (B) fluorescence. The traces were fitted to a single exponential phase and to the sum of two exponential phases, and the corresponding residuals are plotted below the main plot. Experiments were performed in a PBS buffer, 1 mM DTT at 15 °C.