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Specific BSH activity of purified Cm33407BSH. (A) SDS–PAGE of recombinant Cm33407BSH protein (the calculated molecular weight of the recombinant Cm33407BSH is 39.97 kDa). (B) Specific activity of purified BSH from C. minuta DSM33407 on four major primary conjugated bile acids. All experiments were performed in triplicate. Key: GCA: glycocholic acid, GCDCA: glycochenodeoxycholic acid, TCA: taurocholic acid, TCDCA: taurochenodeoxycholic acid.
