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Representation of the functionalization and radiolabeling of the hPD-L1 Nb: (A) Dimerized Nbs are reduced in mild conditions (2-MEA = 2-mercaptoethylamine); (B) Site-specific functionalization of the Nb’s C-terminal cystein-tag with maleimide-NOTA via a Michael addition; (C) Gallium-68 labeling of the NOTA-mal-(hPD-L1) Nb.
