Table 2.
Preparation steps of microtiter plate for the MIC test.
| Wells (column no.) → | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| Stages | Concentrations (%) | 100 | 50 | 25 | 12.5 | 6.25 | 3.13 | 1.56 | 0.78 | 0.39 | 0.19 | Positive control (Broth + Bacteria) | Negative control (Broth) |
| 1 |
Addition of
MH broth (µL) |
- | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| 2 |
Addition of
extract or NPs (µL) |
100 | 100 | Serial two-fold dilutions for wells 3–10 | 0 | 0 | |||||||
| 3 |
Addition of
Bacteria (µL) |
100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 |
| 4 | Addition of resazurin (µL) | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 30 |