Table 1.

List of primary and secondary antibodies used for immunohistochemical analysis.

Primary Antibody	Company	Catalog No.	Pre-Treatment	Detection	Dilution	Secondary Antibody
SOX2	Cell Signaling Technology	2748	Citrate buffer (pH 6.0), 20 min, 98 °C in water bath	IH-P (DAB), Hematoxylin	1:100	VECTASTAIN ABC HRP Kit (Peroxidase, Rabbit IgG), Vector Laboratories, USA, cat. No. PK-4010
AXIN2	Abcam	32197	Citrate buffer (pH 6.0), 20 min, 98 °C in water bath	IH-P (DAB), Hematoxylin	1:200	VECTASTAIN ABC HRP Kit (Peroxidase, Rabbit IgG), Vector Laboratories, USA, cat. No. PK-4010
β-Catenin PY 489	DSHB	10144551	Citrate buffer (pH 6.0), 20 min, 98 °C in water bath	IH-P (DAB), Hematoxylin	1:50	VECTASTAIN ABC HRP Kit (Peroxidase, Mouse IgG), Vector Laboratories, USA, cat. No. PK-4002
Ki-67	Cell Marque	275-R-16	Citrate buffer (pH 6.0), 20 min, 98 °C in water bath	IH-P (DAB), Hematoxylin	1:200	UltraView Universal DAB Detection Kit, Ventana, cat. No. 05269806001