Table 2.

List of primary and secondary antibodies used for immunofluorescence detection.

Primary Antibody	Company	Catalog No.	Pre-Treatment	Detection	Dilution	Secondary Antibody
FZD6	Assay Biotechnology	G260	Citrate buffer (pH 6.0), 20 min, 98 °C in water bath	IF (Alexa Fluor 488), DRAQ5	1:100	Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488, Thermo Fisher Scientific, USA, cat. No. A11008
pan Cytokeratin	Abcam	961	Citrate buffer (pH 6.0), 20 min, 98 °C in water bath	IF (Alexa Fluor 568), DRAQ5	1:1	Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568; Thermo Fisher Scientific, USA, cat. No. A11004