Figure 5.

PZ-2891 and compound 7 at doses used in our biological assays had no direct effects on P. falciparum NF54 growth in vitro. Synchronized asexual stage P. falciparum NF54 strain was grown and treated with medium supplemented with chloroquine as a positive control for parasite killing (156 nM, 78 nM, 39 nM or 19.5 nM), PZ-2891 (1.3 µM, 130 nM, 13 nM or 1.3 nM), compound 7 (62 µM, 6.2 µM, 620 nM or 62 nM) or DMSO as a negative control at a volume equivalent to that used to deliver small molecules (indicated as “Pf control”, set to 1). Samples were collected 48 h and 96 h after treatment, stained with PI and analyzed by flow cytometry. The data are represented as mean parasitemia +/− SEM of the treated cultures relative to Pf control. The assays were replicated twice with distinct parasite cultures.