Figure 10.

Effects of (a) l-borneol, (b) amentoflavone, and (c) diclofenac on p-cresol-mediated LDH release in HepaRG cells (0.4 million cells/well). Cells were pre-treated with vehicle or 0.75 mM l-borneol, 75 μM, amentoflavone, or 100 μM diclofenac for 30 min, then co-treated with vehicle or 0.75 mM p-cresol for 24 h as described in Section 2. LDH release was calculated as the percentage of activity in the cell supernatant to that of the sum of cell supernatant and cell lysates. Data are presented as mean ± standard deviation from n = 8 determinations. * p < 0.05 versus the vehicle control (i.e., the HepaRG differentiation medium) using ANOVA on ranks; ** p < 0.05 versus p-Cresol. ANOVA, analysis of variance; LDH, lactate dehydrogenase.