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. 2021 May 29;11(6):808. doi: 10.3390/biom11060808

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Pictilisib inhibits viral replication in the human chip model. The epithelial and endothelial sites of the human chip model were pre-incubated with 10 µM Pictilisib or solvent control (DMSO) for 30 min prior to infection. PR8M infection (MOI 0.1) was then performed for 30 min on the epithelial site of the model. The chip was incubated with Pictilisib (10 µM) or DMSO to 24 h p.i. (a,b) The cell layers of the epithelial (a) and endothelial chamber (b) were stained using primary antibodies against IV nucleoprotein (a,b), E-cadherin (a) and VE-cadherin (b), Nuclei were stained with Hoechst (a,b). (c) Viral titers were determined by standard plaque assay. The solvent control was arbitrarily set to 100%. Data represents the mean + SD of eight independent experiments. Statistical significance was determined by multiple t-tests (**** p < 0.0001).

Pictilisib inhibits viral replication in the human chip model. The epithelial and endothelial sites of the human chip model were pre-incubated with 10 µM Pictilisib or solvent control (DMSO) for 30 min prior to infection. PR8M infection (MOI 0.1) was then performed for 30 min on the epithelial site of the model. The chip was incubated with Pictilisib (10 µM) or DMSO to 24 h p.i. (a,b) The cell layers of the epithelial (a) and endothelial chamber (b) were stained using primary antibodies against IV nucleoprotein (a,b), E-cadherin (a) and VE-cadherin (b), Nuclei were stained with Hoechst (a,b). (c) Viral titers were determined by standard plaque assay. The solvent control was arbitrarily set to 100%. Data represents the mean + SD of eight independent experiments. Statistical significance was determined by multiple t-tests (**** p < 0.0001).