Fig. 4.

Characterization of MV4F^N-derived induced pluripotent stem cell (iPSC) clones for pluripotency markers. a One representative MV4F^N-derived iPSC clones obtained with or without small molecules ( + sm, −sm) and one 4LV-derived iPSC control (4LV) were cultured under feeder-free conditions on a matrigel-based slide and examined for expression of human pluripotent stem cell markers by immunofluorescence. Passages 2–4 and 20 were analyzed. Scale bars represent 50 μm. b RT-PCR analysis assessing transcription of key pluripotency-associated genes (OCT4, SOX2, KLF4, NANOG, GDF3, hTERT, cMYC) using total cellular RNA of two representative iPSC clones obtained with or without small molecules at passages 3 and 22. GAPDH is the cellular internal control, and water is the negative control. c RT-PCR analysis assessing transcription of viral and cellular genes OCT4, and cMYC were analyzed using total cellular RNA of one representative iPSC clones obtained with or without small molecules at passages 1. GAPDH is the cellular internal control, and water is the negative control. BJ cells and measles virus (MV)-infected BJ cells were used as negative and positive control of viral OCT4 and cMYC