Table 5.

The performance of ctDNA-based sequencing analysis for CCA diagnosis.

Gene	ctDNA Mutations	Tumor Mutations		Sensitivity (%)	Specificity (%)	PPV (%)	NPV (%)	False Positive (%)	False Negative (%)	Diagnostic Accuracy (%)
		+	−
ARID1A	+	3	0	100.00	100.00	100.00	100.00	0.00	0.00	100.00
	−	0	7
PBRM1	+	3	0	100.00	100.00	100.00	100.00	0.00	0.00	100.00
	−	0	7
NCOR1	+	2	0	100.00	100.00	100.00	100.00	0.00	0.00	100.00
	−	0	8
MTOR	+	3	0	100.00	75.00	100.00	85.71	0.00	14.29	90.00
	−	1	6
EZH2	+	2	0	100.00	66.67	100.00	87.50	0.00	12.50	90.00
	−	1	7
PTEN	+	2	0	100.00	66.67	100.00	87.50	0.00	12.50	90.00
	−	1	7
FGFR3	+	2	1	87.50	100.00	66.67	100.00	33.33	0.00	90.00
	−	0	7
RASA1	+	1	0	100.00	33.33	100.00	77.78	0.00	22.22	80.00
	−	2	7
TP53	+	1	1	85.71	33.33	50.00	75.00	50.00	25.00	70.00
	−	2	6
Total	+	19	2	96.88	73.08	90.48	89.86	9.52	10.14	90.00
	−	7	62

Abbreviations; PPV: positive predictive value, NPV: negative predictive value.