Table 2.
Bacterial strains, plasmids, and oligonucleotides
| Bacteria, plasmid, oligonucleotides | Relevant characteristic(s) or sequence | Source | |
|---|---|---|---|
| Bacterial strains | |||
| E. coli DH5αλpir | Plasmid cloning strain | [21] | |
| S. aureus RN4220 | Restriction deficient strain, partially defective AgrA | [43] | |
| S. aureus 10400326 or Sa2 | ST351, AIP-II producer | [44] | |
| S. aureus 10303344 or Sa25 | ST352, AIP-I producer | [44] | |
| S. aureus 41000044 or Sa27 | ST151, AIP-II producer | [44] | |
| S. aureus 21000024 or Sa30 | ST352, AIP-I producer | [44] | |
| L. lactis subsp. lactis ATCC 11454 | Nisin producer | [45] | |
| S. epidermidis ATCC 14990 | AIPep-I producer | [46] | |
| Plasmids | |||
| pBGR1 | Promoter trap vector | [47] | |
| pKK30 | Highly stable plasmid containing PsarAP1-dfrA | [21] | |
| pQS1 | pKK30 containing PagrP3-gfpbk | This study | |
| pQS3 | pKK30 containing PagrP3-mCherry | This study | |
| Primers | |||
| AGRP3-F3 (EcoRI) | AAAGAATTCGTAATTTGTATTTAATATTTTAAC | This study | |
| AGRP3-F4 (NheI) | AAAGCTAGCGTAATTTGTATTTAATATTTTAAC | This study | |
| AGRP3-R1 (BamHI) | AAAGGATCCCAACTATTTTCCATCAC | This study | |
| GFP-R1 (EcoRV) | AAAGATATCTTATTTGTAGAGCTC | This study | |
| mCherry-R1 (BamHI) | AAAGGATCCCTACTTGTACAGCTC | This study | |