Table 2.
PCR primers used in this study.
| Primers | Targeted Genes | Sequence (5′-3′) |
|---|---|---|
| TL17 | M13F | GTAAAACGACGGCCAG |
| TL18 | M13R | CAGGAAACAGCTATGAC |
| TL19 | NEO split F | GGGCGCCCGGTTCTTTTTGTCA |
| TL20 | NEO split R | TTGGTGGTCGAATGGGCAGGTAGC |
| TL59 | NEO R4 | TGTGGATGCTGGCGGAGGATA |
| TL217 | GAPDH Q-PCR F1 | TGAGAAGGACCCTGCCAACA |
| TL218 | GAPDH Q-PCR R1 | ACTCCGGCTTGTAGGCATCAA |
| TL327 | VLP1 KO F1 | GGTCAGGCGTGGAAGCGTCATACA |
| TL328 | VLP1 KO R1 | CTGGCCGTCGTTTTACGCTGCAACAAGTCGCGTCATTTAC |
| TL329 | VLP1 KO F2 | GTCATAGCTGTTTCCTGTCCGCAATGCCACAAGAGACTG |
| TL330 | VLP1 KO R2 | GAGACCCGGGGCCTAATACCTAAT |
| TL331 | VLP1 KO F3 | GCTCACCCCGAAAACAGATACAGG |
| TL332 | VLP1 KO R3 | AAGGCGGGGAGGGAGGATTC |
| TL333 | VLP1 KO F4 | AGCTGTGCCCCATCTTTTTAGTTA |
| TL971 | VLP1 CDS F1 | GACGAGCTGTAcGGATCCATGGCGACCGTCGAGTGCGTGCTG (BamHI) |
| TL972 | VLP1 CDS R1 | CTGGCGGCCGTTACTAGTCTATTCTCTTGAAGCGGCCAAGTT(SpeI) |
| TL1016 | VLP1 Comp F1 | GATATCGAATTCCTGCAGCCCGGGGGATCCGGAAAAGTTAAAAGTCATTGGCAGTT (BamHI) |
| TL1017 | VLP1 Comp R1 | CGGTGGCGGCCGCTCTAGAACTAGTGGATCTTGGAAAAGAAAAGGAAAGTGAAATC (BamHI) |
| TL1018 | VLP1-HA F1 | CGCCCAACATGTCTGGATCCATGGCGACCGTCGAGTGCGTGCTG (BamHI) |
| TL1019 | VLP1-HA R1 | ACGTCGTATGGGTAGGATCCTTCTCTTGAAGCGGCCAAGTTTGC (BamHI) |
| TL1281 | VLP1 Q-PCR F1 | GAAAGGGAGGAAAGAGGAAGTG |
| TL1282 | VLP1 Q-PCR R1 | TATTCTCTTGAAGCGGCCAAG |