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. 2021 May 26;10(6):467. doi: 10.3390/biology10060467

Figure 4.

Figure 4

Shh pathway is only active in U87MG cells, and HDAC6 silencing inhibits this pathway. (A) RT-qPCR for Gli1 in human GBM cell lines and NHA cell line. Data are represented as mean ± SD (****) p < 0.0001 vs. NHA. (B) Gli1-luciferase assay in human GBM cell lines and NHA. Data are represented as mean ± SD (**) p < 0.01 vs. 8× mut (n = 4 of three independent experiments). (C) RT-qPCR for Gli1 in U87MG cell line after silencing of HDAC6. Data are represented as mean ± SD (*) p < 0.05 vs. siCTRL condition (n = 3). (D) Gli1-luciferase assay in U87MG cell line after treatment with siRNAs. Data are represented as mean ± SD (**) p < 0.01 (n = 4 of three independent experiments).