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. 2021 Jun 1;10(6):1122. doi: 10.3390/plants10061122

Figure 8.

Figure 8

G. procumbens induces the migration of human keratinocytes, fibroblasts and endothelial cells under diabetic conditions. Cells were cultured in 38 mM glucose or 38 mM mannitol-containing medium for 24 h and then treated with 10 μg/mL mitomycin C for 2 h. A scratch assay was performed and the wounded monolayers were photographed under phase-contrast microscopy. Representative photographs of (a) primary human keratinocytes, (b) fibroblasts and (c) endothelial cells stimulated with 50 μg/mL G. procumbens (GP) or 0.1% DMSO in normal medium (vehicle) or 0.1% DMSO in high-glucose medium (HG) for 24 h. Right panels: the residual wound area was measured by ImageJ software. The values are presented as the means ± SD of four independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 for the comparisons between vehicle and G. procumbens (GP) in normal medium. & p < 0.05, && p < 0.01 and &&&& p < 0.0001 for the comparison between vehicle (HG) and G. procumbens (HG+GP) in high-glucose medium. # p < 0.05, ## p < 0.01 and ### p < 0.001 for the comparisons between vehicle in normal medium and vehicle in high-glucose medium (HG).