Figure 4.

MICs of selected genes inactivated in BW25113 cells from the Keio collection. MIC determination was performed by varying the concentration of the oxidizing agents, with 1 mM increments from 1 to 10 mM for both H₂O₂ and HOCl and 0.25 mM increments from 0 to 1 mM (if MICs were lower than 1 mM) in LB medium or M9 minimal medium. The lowest concentration at which bacteria could not grow was recorded as the corresponding MIC. Each strain was tested in three biological replicates. Significant differences (p < 0.05) are marked with *, and highly significant differences (p < 0.001) are marked with **.