Figure 3.

Volcano maps of differential metabolites in positive and negative ion modes from colon, duodenum, and rectum tissue samples from the Hyplus rabbits fed either an antibiotic-free diet (Dia group) or a standard normal diet (Con group). Volcano maps can visually display the overall distribution of different metabolites. Abscissas represent multiple differential metabolite changes between the Hyplus rabbit groups (log₂ Fold Change). The ordinate represents the significance levels of differential metabolite changes between the Hyplus rabbit groups (−log₁₀ p-value). Each point in the volcano plots represents a differential metabolite. The red dots represent significantly upregulated metabolites. The green dots represent significantly downregulated metabolites. Dot sizes represent variable importance in projection (VIP) values.