Figure 3.

Heparin facilitates OPN binding to CD44 in surface plasmon resonance experiments. In a BIACORE 2000 instrument, the four channels of a CM5 chip were coated with bovine serum albumin (negative control), GST-OPN, CD44v, or Integrin α₉β₁: (A) Low levels of heparin binding alone to immobilized OPN (top), CD44v (middle), or α₉β₁ (bottom); (B) Binding of heparin, OPN or both in the flow to immobilized OPN (top), CD44v (middle), or α₉β₁ (bottom); (C) Heparin effect on OPN-CD44v binding in ELISA; (D) OPN binding to CD44v in ELISA, following preincubation with heparin for 2 h at the indicated ratios.