Table 3.
Amino acid profile of extracts obtained from subcritical water hydrolysis of codfish frames.
| Amino Acid Profile (mg/g) | ||||||||
|---|---|---|---|---|---|---|---|---|
| 90 °C | 140 °C | 190 °C | 250 °C | |||||
| Free | Total | Free | Total | Free | Total | Free | Total | |
| Arginine (Arg) | 5.22 | 47.14 | 16.67 | 56.85 | 14.06 | 39.74 | 27.83 | 54.75 |
| Lysine (Lys) | 0.19 | 35.01 | 3.77 | 36.50 | 1.15 | 32.53 | 4.91 | 24.54 |
| Alanine (Ala) | 5.40 | 155.56 | 3.31 | 135.56 | 3.07 | 132.41 | 30.67 | 111.41 |
| Threonine (Thr) | n.d. | 4.13 | 2.52 | 4.56 | 1.00 | 9.97 | n.d. | 24.42 |
| Glycine (Gly) | 1.39 | 116.66 | 3.31 | 107.28 | 6.30 | 118.49 | 33.35 | 145.04 |
| Valine (Val) | 0.32 | 10.30 | 2.45 | 15.64 | 2.26 | 10.24 | 7.02 | 3.17 |
| Serine (Ser) | n.d. | 44.93 | 0.40 | 52.48 | 0.54 | 76.51 | n.d. | 81.08 |
| Proline (Pro) | 1.75 | 90.20 | 3.95 | 92.88 | 6.09 | 116.63 | 9.71 | 88.11 |
| Hydroxyproline (Hyp) | 1.59 | 88.91 | 3.55 | 81.61 | 5.56 | 86.12 | 12.07 | 100.27 |
| Leucine (Leu) | 0.18 | 23.78 | 0.89 | 18.66 | 0.60 | 4.29 | 6.53 | 24.52 |
| Isoleucine (Ile) | 0.42 | 20.52 | 2.76 | 30.82 | 3.55 | 59.83 | 14.61 | 37.74 |
| Methionine (Met) | 0.29 | 6.05 | 2.84 | 5.60 | 0.68 | 24.20 | 4.13 | 7.77 |
| Histidine (His) | 0.09 | 8.44 | 4.62 | 4.25 | 0.62 | 5.22 | 1.40 | 4.39 |
| Phenylalanine (Phe) | 0.62 | 5.02 | 4.25 | 18.79 | 3.07 | 4.05 | 3.82 | 3.15 |
| Glutamic acid (Glu) | 0.40 | 30.46 | n.d. | 98.33 | n.d. | 1.10 | 0.00 | 22.69 |
| Aspartic acid (Asp) | 0.32 | 26.63 | 1.86 | 18.39 | 4.45 | 5.31 | 4.45 | 12.67 |
| Cysteine (Cys) | n.d. | n.d. | n.d. | 0.86 | n.d. | 0.70 | n.d. | 0.98 |
| Tyrosine (Tyr) | 0.49 | 5.26 | 3.09 | 42.77 | 3.51 | 6.31 | 5.16 | 8.70 |
| Total | 18.67 | 719.00 | 60.24 | 821.83 | 56.50 | 733.65 | 165.67 | 755.40 |
| Free/total ratio | 0.026 | 0.073 | 0.077 | 0.22 | ||||
Experimental uncertainty (u) = ±0.1 mg/g extract; n.d.: not detected.