Table 2.
Minimum inhibitory concentration (MIC) in µM.
| Bacteria | Growth Media or Ratio | optP7 | c-optP7 | [L6-optP7]Mco | Melittin * |
|---|---|---|---|---|---|
|
P. aeruginosa (Gram-negative) |
MH | 3 | 142 | 62 | ND |
| MH + 10% serum | 49 | >142 | >62 | ND | |
| Ratio MH + 10% serum/ MH | 16.3 | NA | NA | NA | |
| 1/5 MH | 0.8 | 9 | 8 | ND | |
| 1/5 MH + 10% serum | 6 | 35 | 8 | ND | |
| Ratio 1/5 MH + 10% serum/ 1/5MH | 7.5 | 3.9 | 1 | NA | |
|
E. coli (Gram-negative) |
MH | 0.8 | 71 | 62 | 16 |
| MH + 10% serum | 6 | >142 | >62 * | ND | |
| Ratio MH + 10% serum/ MH | 7.5 | NA | NA | NA | |
| 1/5 MH | 0.4–0.8 | 4–9 | 2 | 3 | |
| 1/5 MH + 10% serum | 0.8 | 18 | 15 | 11-22 | |
| 1/5 MH + 10% HI serum | 0.8 | 35 | 15 | 11 | |
| Ratio 1/5 MH + 10% serum/ 1/5MH | 2–1 | 4.5–2 | 7.5 | 3.6–7.3 | |
|
K. pneumoniae (Gram-negative) |
MH | 1.5 | >142 * | 62 * | ND |
| MH + 10% serum | ND | ND | ND | ND | |
| Ratio MH + 10% serum/ MH | NA | NA | NA | NA | |
| 1/5 MH | 1.5 | 71 | 31 | ND | |
| 1/5 MH + 10% serum | 24 | 142 | >62 | ND | |
| Ratio 1/5 MH + 10% serum/ 1/5MH | 16 | 2 | NA | NA | |
|
A. baumannii (Gram-negative) |
MH | 1.5 | ND | 62 | ND |
| MH + 10% serum | ND | ND | ND | ND | |
| Ratio MH + 10% serum/ MH | NA | NA | NA | NA | |
| 1/5 MH | 0.8 | 4 | 4 | ND | |
| 1/5 MH + 10% serum | 3 | 71 | 31 | ND | |
| Ratio 1/5 MH + 10% serum/ 1/5MH | 3.75 | 17.8 | 7.75 | NA | |
| VRE (Gram-positive) |
BHI | 0.8 | >142 * | >62 * | ND |
| BHI + 10% serum | ND | ND | ND | ND | |
| Ratio BHI + 10% serum/ BHI | NA | NA | NA | NA | |
| 1/5 BHI | 0.8 | 18 | 15 | ND | |
| 1/5 BHI + 10% serum | 24 | >142 | 62 | ND | |
| Ratio 1/5 BHI + 10% serum/ 1/5BHI | 30 | >7.8 | 4.1 | NA | |
| MRSA (Gram-positive) |
MH | 6 | 142 | >62 | 4 |
| MH + 10% serum | 49 | >142 | >62 | ND | |
| Ratio MH + 10% serum/ MH | 8.1 | NA | NA | NA | |
| 1/5 MH | 1.5 | 18 | 8 | 0.7 | |
| 1/5 MH + 10% serum | 6 | >142 | >62 | 1.4 | |
| 1/5 MH + 10% HI serum | 6 | >142 | >62 | 1.4 | |
| Ratio 1/5 MH + 10% serum/ 1/5MH | 4 | >7.9 | >7.8 | 2 |
Data was obtained from experiments performed in triplicate, with modal values reported; asterisk represents a single measurement; serum = human serum; HI serum = heat inactivated human serum; ND = not determined; when calculation of ratio was not possible it was labelled as NA = not applicable; MRSA = methicillin-resistant Staphylococcus aureus; VRE = vancomycin-resistant Enterococcus faecalis; MH = Mueller – Hinton broth; BHI = brain heart infusion broth.