Phylogenetic tree of chloroplast tRNAs. A, alanine (Ala); R, arginine (Arg); N, asparagine (Asn); D, aspartate (Asp); C, cysteine (Cys); Q, glutamine (Gln); E, glutamate (Glu); G, glycine (Gly); H, histidine (His); I, isoleucine (Ile); L, leucine (Leu); K, lysine (Lys); M, methionine (Met); F, phenylalanine (Phe); P, proline (Pro); S, serine (Ser); T, threonine (Thr); W, tryptophan (Trp); Y, tyrosine (Tyr); V, valine (Val). The green solid circle represents Gossypium; the red solid triangle represents P. taeda; the pink solid diamond represents A. lyrate; the yellow solid triangle represents P. nudum; the orange solid triangle represents D. hirsuta; and the blue solid square represents Nostoc sp. PCC 7524. Phylogenetic analysis showed the polyphyletic origin of chloroplast tRNAs.