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Size of sub-visible aggregates formed upon 75 °C heat incubation. The presence of sub-visible aggregates was analyzed by measuring the Z-ave hydrodynamic radius (Rh) using dynamic light scattering (DLS). After 75 °C heat incubation, the samples were centrifuged, and 100 µL of the supernatant was used for DLS measurement at 25 °C. The Rh of VHH-7D12 variants in (A) 20 mM Na-acetate buffer (B) 20 mM phosphate buffer (C) PBS and (D) 20 mM Tris-HCl.
