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. 2021 May 29;11(6):810. doi: 10.3390/biom11060810

Table 2.

Amount of protein (%) retained in the supernatant after thermal stress.

Variants 60 °C 75 °C
Na-Acetate PB PBS Tris-HCl Na-Acetate PB PBS Tris-HCl
VHH-7D12 68.9 89.0 96.1 90.2 73.7 65.9 70.9 74.7
7D12 + Arg * 47.3 91.8 91.6 67.8 90.1 64.2 87.7 52.6
7D12 + Arg ** 96.7 97.0 98.2 97.8 92.0 95.3 97.0 96.1
7D12-C5R 99.8 87.3 80.2 100.0 98.4 62.3 44.6 99.4
7D12-C9R 99.8 89.5 82.8 98.9 97.2 69.3 55.7 100.0
7D12-C5K 98.2 98.8 98.4 100.0 99.0 95.5 96.6 99.4
7D12-C9K 100.0 98.9 99.4 100.0 98.0 98.2 96.2 99.6

* 300 µM free L-arginine ** 500 mM free L-arginine.