Table 2.
Variants | 60 °C | 75 °C | ||||||
---|---|---|---|---|---|---|---|---|
Na-Acetate | PB | PBS | Tris-HCl | Na-Acetate | PB | PBS | Tris-HCl | |
VHH-7D12 | 68.9 | 89.0 | 96.1 | 90.2 | 73.7 | 65.9 | 70.9 | 74.7 |
7D12 + Arg * | 47.3 | 91.8 | 91.6 | 67.8 | 90.1 | 64.2 | 87.7 | 52.6 |
7D12 + Arg ** | 96.7 | 97.0 | 98.2 | 97.8 | 92.0 | 95.3 | 97.0 | 96.1 |
7D12-C5R | 99.8 | 87.3 | 80.2 | 100.0 | 98.4 | 62.3 | 44.6 | 99.4 |
7D12-C9R | 99.8 | 89.5 | 82.8 | 98.9 | 97.2 | 69.3 | 55.7 | 100.0 |
7D12-C5K | 98.2 | 98.8 | 98.4 | 100.0 | 99.0 | 95.5 | 96.6 | 99.4 |
7D12-C9K | 100.0 | 98.9 | 99.4 | 100.0 | 98.0 | 98.2 | 96.2 | 99.6 |
* 300 µM free L-arginine ** 500 mM free L-arginine.